Application of Edman Degradation N-Terminal Sequencing in Analytical Protein and Peptide Sequences
The N-terminal amino acid sequence of a protein is one of the key quality attributes of a biopharmaceutical. Edman degradation N-terminal sequencing is a routine method for analyzing this property. Analysis of the 15 amino acid sequence of a N-terminal by Edman degradation is usually a mandatory item in biopharmaceutical declaration s. It is also an annual inspection project for many listed biopharmaceuticals). In scientific research, proteins with unknown or uncertain theoretical sequences can be used to provide critical information for confirmation using N-terminal sequencing. Therefore, this method has been widely used in scientific research and industry. W hat is its principle ? H ow to analyze the results, and what are the precautions for sample storage ? Principle It can be explained from the following two aspects. Edman degradation : Through the reaction between PITC (phenyl isothiocyanate) and α -amino group o f the protein or N-terminus polypeptide, t