MS Analysis Expert

As shown below, the reporter group whose  mass is 114 contains one  C13 ,  and the mass of the reporter group is increased by 1 Da. At the same time, the balance group contains one  C13 and one  O18, and the total mass is increased by 3 Da, so that the total mass of the 114 label is increased by 4 Da. By analogy, the reporter group of 115   increase s   by 2   Da, the balance  group will increase by 2   Da .   T he reporter group of 116 increase s  by 3   Da, the balance  group will increase by 1   Da .   T he reporter group of 117 increase s  by 4Da, the balance  group will increase by 0   Da.  After t he above four tags are labeled with different isotopes for its  reporter group   and balance group, the respective report er  group and balance group have different masses . However, as the total mass   added   is the same,   the total mass of...

Introduction to iTRAQ and TMT T ag Speaking of iTRAQ  ( Isobaric Tag for Relative and Absolute Quantitation )  and TMT  ( Tandem Mass Tag ) , many of us may think that they are  two differen t quantitative pr o teo mics t echniques, but in fact, except the difference s  in labeling specifications and label molecular structure  as well as developer , the principle of their labeling peptides are b asically the same. iTRAQ was firstly developed by AB SCIEX ,  followed by whcih TMT  was developed by Thermo Fisher .   Although they have  different patents, the y   use almost the same   principle. Th e iTRAQ and TMT  marker a re essentially a chemical in vitro  labeling reagent that specifically labels peptides produced by protein digestion. The iTRAQ and TMT-labeled protein samples are available in different sizes. In contrast, TMT can label a wider range of samples and simultaneously quantify multiple sets of 2 p...