MS Analysis Expert

With the development of proteomics and biomass spectrometry, large scale quantitative proteomics has become an important aspectof proteomics. Quantitative proteomics  plays important roles in deciphering the biological progress, understanding the development of the disease, and discovering as well as verifying biomarkers for the diagnosis and therapy of diseases. The common quantitative proteomics services are label free and stable isotope labeled quantification , eg. SILAC, iTRAQ. Quantitative p roteomics based on Label Free Label   free  quantification is a method in mass spectrometry that aims to determine the relative amount of proteins in two or more biological samples. Label-free approaches to quantitative proteomics have   gained prominence in recent years since no additional   chemistry or sample preparation steps are required. M ethods :  comparing the ratios of integrated   peak areas/intensities of phosphorylated to nonphosphory...

SILAC  quantification is suitable for the analysis of living cultured cells and can be used for the identification and quantification of whole protein and membrane proteins. SILAC is mainly quantified by MS1, and iTRAQ / TMT  is mainly quantified by MS2. There is not much difference in quantitative accuracy and protein resolution between the two, but due to the complicated operation, long time span and high cost required by SILAC technology implementation, it has not been widely commercialized. Label-free , as its name implies, is a method for the identification and quantification of protein enzymatic peptides by non-labeled quantification. It is not necessary to use a reagent containing a stable isotope label. It is only necessary to compare the intensity of MS1 signal between the two groups of samples in a specific peptide to obtain a change in the amount of protein expression between the samples, which is usually used for the analysis of mass spectral data produce...

The proteome refers to the full set of proteins expressed by an organism. Proteomics essentially refers to studying the characteristics of proteins on a large scale, including the expression level of proteins, post   translational modification , protein-protein interactions, etc., thereby obtaining overall and comprehensive understanding of   disease occurrence, cell metabolism  and other processes at protein level . The emergence and development of biological mass spectrometry provide a   reliable and dynamic range of research tools fo r quantitative proteomic s  analysis.    Currently , there are five mainstream   quantitative proteomic s  met hods ,   l abel-free, iTRAQ , SILAC , MRM  (MRMHR), and SWATH . Label-free Label Free Workflow Label   free   quantitative proteomics   does not require specific labeling of comparative samples. It is only necessary to compare the chromatographic mass-spectrom...