Overview of Single/Mixed Protein Separation and Purification Methods and Mass Spectrometry Identification
In terms of common identification method of a single protein, you may immediately think of Western Blot. Western Blot identifies a target protein by specific binding of the antibody to the target molecule , but this method is suitable for identifying known proteins and must be applied to antibodie s, and is not suitable for proteins with unknown or without corresponding antibodies. W hat if the protein to be studied happens to be unknown or has no antibody available? In this case, the protein can be identified by means of mass spectrometry. Single P rotein S eparation and P urification When it comes to single protein sample, as the name suggests, this type of protein sample should have undergone a certain protein purification step to obtain a relatively single protein. Generally, p roteins synthesized by cell lysis or in vitro contain other heteroproteins, so it is necessary to distinguish the target protein from the heteroprotein. The...