MS Analysis Expert

The N-terminal amino acid sequence of protein is one of the key quality attributes of biological drugs. N-terminal sequencing by Edman degradation  is a routine method to analyze this property. The analysis of 15 amino acid sequences on the N-terminal by Edman degradation method is usually a necessary item when applying for biological drugs . At  the same time, it is also an annual inspection item of many listed biological drugs (see Pharmacopoeia 2015). In scientific research, N-terminal sequencing can be used to provide key information for the confirmation of unknown or uncertain theoretical sequences. So this method is widely used in scientific research and industry.  W hat are its principles ? H ow to analyze the results ? Advice for sample delivery? P rinciples In short, we can understand it from these two aspects: The first step  is Edman degradation . T hrough the reaction of PITC (Phenylisothiocyanate), an organic reagent, and α - amino acid at t...

Th e N-terminal amino acid sequence of a protein is one of the key quality attributes of a biopharmaceutical. Edman degradation N-terminal sequencing is a conventional method for analyzing this property. Analysis of the 15 amino acid sequences at the N-terminus by the Edman degradation method is usually a mandatory inspection item when applying for biopharmaceutical s. It  is also an annual inspection item for many biopharmaceuticals already on the market. In scientific research, N-terminal sequencing can be used for proteins with unknown or uncertain theoretical sequences to provide key information for confirmation. Therefore, this method is widely used in scientific research and industry. While,  what is its principl e, how to analyze the results, and what are the suggestions for sending samples? To put it simply, we can understand from these two aspects: Step I:  Edman degradation . T he organic reagent of PITC (benzene isothiocyanate) reacts with the α-amino ...

The N-terminal amino acid sequence  of a protein is one of the key quality attributes of a biopharmaceutical. Edman degradation   N-terminal sequencing  is a routine method for analyzing this property. Analysis of the 15   amino acid sequence  of a N-terminal   by Edman degradation is usually a mandatory item in biopharmaceutical declaration s. It  is also an annual inspection project for many listed biopharmaceuticals). In scientific research, proteins with unknown or uncertain theoretical sequences can be used to provide critical information for confirmation using N-terminal sequencing. Therefore, this method has been widely used in scientific research and industry. W hat is its principle ? H ow to analyze the results, and what are the precautions for sample storage ? Principle It can be explained from the following two aspects. Edman degradation : Through the reaction between PITC (phenyl isothiocyanate) and α -amino group  o f the...