MS Analysis Expert

In the field of proteomics research, liquid chromatography-mass spectrometry (LC-MS/MS)-based discovery proteomics can detect and relatively quantify thousands of proteins in biological samples over the past decade or two. It has been widely used in various research, and reseaechers have developed two mainstream methods, label quantification (iTRAQ/TMT, SILAC) and label free, which are based on data-dependent acquisition (DADA). The method of collecting protein spectral data is also referred to as "shotgun". However, the DDA data acquisition mode has inherent shortcomings such as poor repeatability, inaccurate quantification, large data loss, and difficulty in detecting low abundance proteins. MRM /SRM/PRM-based target proteomics can provide accurate absolute quantitative results for tens to hundreds of target proteins with high repeatability and is the gold standard for absolute quantitation of mass spectrometry. However, the flux is relatively low, and accurate quantitati...

Delayed  and unscheduled Alzheimer's disease accounts for 99% of all such diseases, and it is associated with a variety of pathogenic factors and pathological mechanisms. One of them is a post-translational modification  process of proteins called "O-linked beta-N acetylglucosamine modification" or "O-GlcNAc". The low content and easily decomposable properties of O-GlcNAc make it difficult to study this type of protein modification, and it is also difficult for scientists to quantitatively study O-GlcNAc on a large scale. According to an article published in the Journal of Pathology , researchers used a new approach to integrated proteomics to study this type of protein modification in detail. The author of the article is Cheng-Xin Gong from the City University of New York and Sheng Wang from the Pacific Northwest National Laboratory. This study systematically quantified proteomic analysis of the characteristics of O-GlcNAc modification in the...