MS Analysis Expert

L ung cancer mortality ranks first among all malignancies w orldwide , and the survival rate is quite low. Currently, t here are two methods for diagnosis, low-dose spiral CT (LDCT) (high false positive rate and radiation damage)  and tumor tissue testing (gene mutation screening) . T here is no non-invasive clinical diagnosis mark  to  guide the treatment  for now . The  research paper ( Non-invasive urinary metabolomic profiling identifies diagnostic and prognostic markers in lung cancer , Cancer Research   IF=9.3215 ) introduced today aims to find biomarkers for lung cancer diagnosis by performing metabolomics tests on urine . There are three main reasons why this paper can get a good score, great samples, techniques and data analysis. Samples Used in the Experiment Pre-screening samples (1005 cases): samples collected in 10 years(1998~2007)  from 469 patients (urine  collected before the treatment) and  536 healthy people . ...

Review on UHPLC-Q-TOF/MS B ased plasma M etabolomics R eveals   the M etabolic P ertur b ations by M anganese E xposure in R at M odels Research Background Manganese is a trace metal element necessary for human  beings  and other animals, but  the accumulat ion of high concentrations of manganese   in the brain  can cause manganese toxicity. At present, there is no biomarker can be used as the gold standard for judging manganese toxicity. Therefore, this paper us es un targeted metabolomics to find bio markers for determining manganese toxicity from the level of metabolites. Sample Source Rat plasma (experimental group) fed for 5 weeks with 200 mg/L manganese and rat plasma (control) fed without manganese for 5  weeks. Technical Route untargeted metabolomics  ( UHPLC-Q-TOF/MS , see fig. below) Research R esult Manganese toxicity can cause liver damage in rats: Manganese treatment reduces the ratio of rat live...

Metabolomics is a newly developed subject following genomics and proteomics. It analyzes the relationship between metabolites and physiological as well as  pathological changes in humans through accurate qualitative and quantitative quantification  of small molecule metabolites (50~1,500 Da) in human body , in order to  study the occurrence and development of diseases, find biomarkers of diseases, and to predict the prognosis of diseases. Metabolomics has  broad prospects in clinical diagnosis. There are four  main application s, application in clinical diagnosis (Biomarker), research and application in etiology and pathological mechanism, clinical diagnosis,  etiology and pathological mechanism study, clinical medication guidance and preclinical animal model screening. Application in Clinical D iagnosis In gener al, un targeted metabolomics  tech niques are firstly used to compare all metabolites in the control and experimental groups and...