Techniques for Quantitative Proteomics
With the development and maturity of mass spectrometry, the technical advantages of high throughput, high sensitivity and wider dynamic range have been emerged, which provides a reliable research method for proteomics research. In recent years, quantitative proteomics has become one of the research hotspots in omics field. Quantitative proteomics is an emerging technology used for accurate quantification and identification of proteins expression in biological samples. It can not only identify the proteins expressed in different states, but also accurately quantify their abundance. According to different quantitative purposes, quantitative proteomics can be divided into two categories relative quantitative proteomics and absolute quantitative proteomics. Relative quantitative proteomics, also known as comparative proteomics, is a comparative analysis of protein expression level in cells, tissues or organism in different physiological or pathological conditions. Absolute quantitative pr