Principles and Methods of Mass Spectrometry Analysis of Proteins
Mass spectrometry is sensitivity and accura te , and can accurately identify proteins. At present, mass spectrometry mainly identifies the primary structure of egg, including molecular weight, amino acid sequence of peptide chain and number and position of polypeptide or disulfide bond, which plays an important role in the study of protein structure analysis. Mass spectrometry consists of injector, ion source, mass analyzer, ion detector, control computer, and data analysis system. Traditional mass spectrometry is only used for the analysis of small molecule volatiles, but with the emergence of new ionization techniques such as matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and electrospray ionization mass spectrometry (ESI-MS) , e tc . Th e emergence of various mass spectrometry techniques provides a new and accurate way for protein analysis . Now, the combined application of enzymatic hydrolysis, liquid chromatography separation, tan