Mass Spectrometry Analysis Expert provides the latest proteomics, metabolomics information based on years of experience in this field.
Protein Data-independent Acquisition Quantitative Technology
Get link
Facebook
X
Pinterest
Email
Other Apps
In the field of proteomics research, liquid chromatography-mass spectrometry (LC-MS/MS)-based discovery proteomics can detect and relatively quantify thousands of proteins in biological samples over the past decade or two. It has been widely used in various research, and reseaechers have developed two mainstream methods, label quantification (iTRAQ/TMT, SILAC) and label free, which are based on data-dependent acquisition (DADA). The method of collecting protein spectral data is also referred to as "shotgun".
However, the DDA data acquisition mode has inherent shortcomings such as poor repeatability, inaccurate quantification, large data loss, and difficulty in detecting low abundance proteins. MRM/SRM/PRM-based target proteomics can provide accurate absolute quantitative results for tens to hundreds of target proteins with high repeatability and is the gold standard for absolute quantitation of mass spectrometry. However, the flux is relatively low, and accurate quantitation of high-throughput proteins cannot be performed.
Is there a technology, which is not only of high-throughput, quantitative,but with accurate repeatability as well? The DIA technology (data-independent acquisition) developed and rapidly ignited in recent years is such the technology.
This technology combines the high-throughput and mass spectrometry of the traditional proteome shotgun with the quantitative and accurate advantages of the absolute quantitative gold standard MRM/PRM, which is named the most noteworthy technology of 2015 by Nature Methods. The number of published articles related to DIA has grown rapidly in the past three years and is the current fragrance in the field of proteomics research.
Protein DIA Quantitative Publication Trend (data source: Pubmed) DIA: DIA/SWATH The entire scanning range of the mass spectrum is divided into several parts, and all the parent ions in each part are selected, fragmented, and detected at high speed and cyclically, and all pieces of information of all the parent ions in the sample are obtained without any deviation.
Protein DIA Quantitative Analysis Workflow Applications of Protein DIA Quantitative Analysis: Medicine, botany, pharmacy, animal husbandry, food and environment.
With the development and maturity of mass spectrometry, the technical advantages of high throughput, high sensitivity and wider dynamic range have been emerged, which provides a reliable research method for proteomics research. In recent years, quantitative proteomics has become one of the research hotspots in omics field. Quantitative proteomics is an emerging technology used for accurate quantification and identification of proteins expression in biological samples. It can not only identify the proteins expressed in different states, but also accurately quantify their abundance. According to different quantitative purposes, quantitative proteomics can be divided into two categories relative quantitative proteomics and absolute quantitative proteomics. Relative quantitative proteomics, also known as comparative proteomics, is a comparative analysis of protein expression level in cells, tissues or organism in different physiological or pathological conditions. Absolute quantitativ...
Q11 . In which journals are proteomics experiment related articles ususlly published ? W hat are the difference s ? MCP (M olecular & C ellular P roteomics ): impact factor 7 JPR (journal of proteome research ): impact factor 5 Proteomics : impact factor 5 Journal of Prteomics : impact factor 5 Electrophoresis : impact factor 5 Plos One : impact factor 5 Other magazines : BMC Genomics, Expert Rev Proteomics, BMC Syst Biol, OMICS, Proteomics Clin Appl, Proteome Sci , etc. Q12 . What techniques need to be repeated ? Which one is used in the repeated experiment, biological repetition or technical repetition? Label free needs to be repeated for three times, so do iTRAQ and TMT . If a large number of verification experiments such as WB or ELISA are designed in the experiment, it is better to repeat them twice. If the experiment is not repeated and there are onl...
Comments
Post a Comment